This experiment was carried out to perform staining methods on microbes, explain the mechanisms of staining, namely, simple staining, and learn how to use the microscope. We performed a two-part experiment to gain a better understanding of bacteria through gram-staining gram staining is a differential staining method which results in the classification of two groups of bacteria, gram-positive and gram-negative. The endospore stain endospore production is a very important characteristic of some bacteria, allowing them to resist adverse environmental conditions such as desiccation, chemical exposure. Staining techniques in microbiology 1 staining techniques in microbiology a presentation by g prashanth kumar department of microbiology & parasitology, faculty of medicine, international medical & technological university, dar-es-salaam, tanzania. The first step in most bacterial staining procedures is the preparation of a smear in a smear preparation, in a smear preparation, cells from a culture are spread in a thin film over a small area of a microscope slide, dried, and then fixed.
Lab report: no formal report: gram stain results, antibiotic sensitivity results, bacterial plate counts and calculations are requried remember from the gram stain is used to differentiate bacteria into two groups based on cell color after staining a standard part of nearly all lab courses in introductory microbiology is an activity then. Lab report: differential staining name university date lab report: differential staining abstract the prime focal point of a differential staining lab experiment is on simple stains and microscopy differential staining relies on the bacterial cell wall structure in order to differentiate the gram negative from the gram positive the latter has. I just performed a lab on differential staining, we did a lab on gram staining, acid fast staining, and spore staining one of the questions in the lab was why were the bacteria that were used for each experiment used.
Amanda ratliff lab report #1 3-5-12 gram staining lab report introduction: to better understand and recognize the difference between gram positive and please report to room 311 on the third floor at 9:00 am sharp dilution on a table in the laboratory report section 11 due: gram staining. View lab report - differential staining lab report revised from scibls 101 at north carolina state university harrington 1 differential staining of escherichia coli and staphylococcus epidermidis. Mgh poct inter-laboratory correlation procedure 07-14-10, lab report 10-2-10, lab report 07-7-10, lab report, gram stain of csf secondary to suspected diagnosis of meningitis gram stain is one of the most common staining procedures.
Introduction to lab ex differential stains gram staining home documents introduction to lab ex differential stains gram staining please download to view. Gram staining from wikipedia, the free encyclopedia a gram stain of mixed staphylococcus aureus (staphylococcus aureus atcc 25923, gram-positive cocci, in purple) andescherichia coli (escherichia coli atcc 11775, gram-negative bacilli, in red), the most common gram stain reference bacteria gram staining (or gram’s method. Aim to perform capsule staining of bacteria, to observe bacterial capsule purpose: in some bacteria, the cell wall is surrounded by a viscous cell envelope called ‘capsule’ it is made of polysaccharide, glycoprotein or polypeptide. A the gram stain discussion the gram stain is the most widely used staining procedure in bacteriology it is called a differential stain since it differentiates between gram-positive and gram-negative bacteriabacteria that stain purple with the gram staining procedure are termed gram-positive those that stain pink are said to be.
Gram stain report introduction the purpose of this experiment is to determine the shape and gram stain of the bacteria under a microscope the reason for staining bacteria is due to the fact that most bacteria are transparent and cannot be seen through the microscope the gram stain is a type of differential stain that allows a (micro)biologist. Gram staining lab report by structure of essay writing • 2017-09-27t20:07:39+03:00 a drop of deionized water was placed on a microscope slide and bacteria from unknown number 20 were also placed on the slide • differential stain learn vocabulary, terms, and more with flashcards, games, and other study tools as with gram stains. Click to download word version objective the objective of this lab was to perform various tests using knowledge learned in class, to decipher an unknown culture by utilizing different agar plates and test tubes to select. Mcb 3020l lab experiment 2 the gram stain a one lab session experiment in most in 1883 hans christian gram invented an important differential staining method that is extensively used today the stain is called the gram stain this staining procedure differentiates microbes into two basic groups: gram positive microbes and gram negative microbes differential. For freshman/sophomore courses in general introductory microbiology, and microbiology for allied health students this laboratory manual serves as a general introduction to the microbiology laboratory, including basic procedures and equipment.
Gram staining the pathology lab report was also negative put on gloves and tie back long hair to prevent contaminating the bacteria sample you'll be testing introduction gram staining is the most essential and universally used staining technique in bacteriology laboratory page 1 of 2 treat and report the client based on the gram stain results alone it is a differential. The complete blood cell count (cbc) cbc – part 2 wbc differential count and morphology louisiana state university health sciences center department of pathology. The differential gram stain gives additional information about the bacterial cell wall, which may be gram positive (the deep purple color of crystal violet), or gram negative (the pink color of the counterstain safranin) this difference in staining reflects a fundamental difference in. Lab exercise 9: differential stains for visualization of cell structures objectives 1 understand the chemical basis for the spore and capsule stains 2 perform a successful spore stain to differentiate between bacterial spores and vegetative cells 3 perform a successful capsule stain to distinguish capsular material from the bacterial cell.
Lab 2: staining and streaking protocols for simple stain, gram stain, streak plate technique and culture maintenance lab 2a: introduction to staining live specimens are difficult to see with the bright field microscope. Gram staining: principle, procedure and results february 2, 2015 tankeshwar bacteriology, staining techniques in microbiology 47 gram staining method, the most important procedure in microbiology, was developed by danish physician hans christian gram in 1884 gram staining is still the cornerstone of bacterial identification and. Lab report of microbiology 3 uploaded by akbar haqi introduction gram staining is the most essential and universally used staining technique in bacteriology laboratory gram-staining was firstly introduced by cristian gram in 1883this method is used to distinguish between gram positive and gram-negative bacteria which have. Acid fast stain the acid-fast stain is a differential stain used to identify acid-fast organisms such as members of the genus mycobacterium acid-fast organisms are characterized by wax-like, nearly impermeable cell walls they contain mycolic acid and large amounts of fatty acids, waxes, and complex lipids.
Lab experiment #5-differential staining through the process of differential staining, there are distinct differences between the cell walls of gram-positive and gram-negative bacteria in the case of gram-positive bacteria, the cell wall is comprised of 60-90% peptidoglycan and is very thick there are numerous layers of teichoic acid bound. 3 laboratory report sheet questions: 1 record your results of staining e coli staphylococcus 2 since everything on the slide will be the same color in simple staining, what you.